Cell division cycle associated 8 (BOR, Borealin, BOREALIN, Cell division cycle-associated protein 8, Dasra-B, FLJ10468, FLJ12042, hDasra-B, PESCRG3, Pluripotent embryonic stem cell-related gene 3 protein)

CDCA8 is a component of a chromosomal passenger complex (CPC) required for stability of the bipolar mitotic spindle. The chromosomal passenger complex, which includes Survivin, CDCA8, INCENP and Aurora-B, is known to play crucial roles during mitosis and cell division. It was found that CDCA8 interacting with Aurora-B, INCENP and Survivin, increases during G2/M phase and then reduces after exit from M phase. CDCA8 is cell cycle regulated, down-regulated in response to p53/Rb-signaling, and up-regulated in many types of cancerous tissues. In Drosophila cells, inactivation of CDCA8 results in polyploidy, delayed mitosis and abnormal tissue development, indicating its critical role for cell proliferation. Recent studies show that CDCA8 is essential for cell proliferation during early embryonic development, and its early embryonic lethality cannot be rescued by the loss of p53. Its aberrant expression is linked to a poor prognosis for gastric cancer.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: Rat Kidney Tissue Lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C2098-69B

Size

100ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS containing 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

References

1. Gassmann R, Carvalho A, Hanzing AJ, et al. Borealin: a novel chromosomal passenger required for stability of the bipolar mitotic spindle. J. Cell Biol. 2004; 166:179-91. 2. Tani M, Okino N, Mitsutake S, et al. Molecular Cloning of the Full-length cDNA Encoding Mouse Neutral Ceramidase. J. Biol. Chem. 2000; 275:11229-34. 3. Hanson KK, Kelley AC, and Bienz M Loss of Drosophila borealin causes polyploidy, delayed apoptosis and abnormal tissue development. Development 2005; 132:4777

Additional Info

Recognizes human CDCA8. Species Crossreactivity: Mouse and rat.