CLEC5A is a type II membrane protein belonging to the C-type lectin superfamily. CLEC5A is expressed on monocytes/macrophages, dendritic cells, and NK cells and contributes to cellular activation through its association with DAP12.
Applications:
Suitable for use in Flow Cytometry. Other applications not tested.
Recommended Dilutions:
Flow Cytometry: 10ul labels up to 1x10e6 cells.
Optimal dilutions to be determined by the researcher.
Positive Control
Whole blood monocytes.
Flow Cytometry Protocol:
1. Cells may be Fc-blocked with 1ug of human IgG/10e5 cells for 15 minutes at RT. Do not wash excess blocking IgG from this reaction.
2. After blocking, add 10ul of C5839-75 to up to 1x10e6 cells and incubate for 30 minutes at RT.
3. Remove unbound antibody by washing the cells twice in Flow Cytometry Staining Buffer (F4568-10). Note that whole blood requires a RBC lysis step at this point using Flow Cytometry Human Lyse Buffer.
4. Resuspend the cells in Flow Cytometry Staining Buffer for final flow cytometric analysis. As a control for this analysis, cells in a separate tube should be treated with APC-labeled mouse IgG2B antibody.
Storage and Stability:
May be stored at 4 degrees C before opening. DO NOT FREEZE! Stable at 4 degrees C Stable for 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Freezing APC conjugates will result in a substantial loss of activity. APC is light sensitive.
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