Cytokeratin 18

Cat no: C9097-20B

Supplier: United States Biological

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Description
Additional Information

Cytokeratin 18 (CK-18), a normal constituent of the hepatocyte cytoskeleton) is a standard tumor marker. CK-18 is elevated in patients with non-malignant liver diseases, particularly in those with prominent cytolysis (1). CK-18 and the homologous mouse Endo B are type I IF protein subunits whose expression is restricted in adults to a variety of simple epithelial tissues. Keratin 8 and 18 (CK8/18) are the major keratin pair in simple-type epithelia, as found in the liver, pancreas, and intestine. Transgenic mice that express mutant keratin 18 develop chronic hepatitis, and have an increased susceptibility to drug-induced hepatotoxicity (2). Studies shows that high CK18 expression in tumor cells is associated with reduced invasiveness in vitro and lack of tumorigenicity in nude mice. It has been shown that high K18 expression correlated with a good prognosis and that reducing K18 expression increased the aggressiveness of established breast cancer cell lines (3). Applications: Suitable for use in Flow Cytometry, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:15 Western Blot: 1:50,000 Immunoprecipitation: 1:100 Immunohistochemistry: 1:250-1:500 Immunocytochemistry: 1:250-1:500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Catalogue number:	C9097-20B
Reactivities:	Human
Hosts:	Rabbit
Applications:	ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Size:	100ul
Form:	Supplied as a liquid in 50 mM Tris-Glycine (pH 7.4), 0.15 M sodium chloride, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
P type:	Pab
Isotype:	IgG
Purity:	Purified by immunoaffinity chromatography.
Alternative names:	CK 18, CK18, CYK18, Cytokeratin endo B, K18, Keratin 18, Keratin D, Keratin type I cytoskeletal 18, Kerd, KRT18
References:	1. Gonzalez-Quintela A, et al. Liver Int 26(10):1217-24, 2006. 2. Ku, NO, et al. J. Clin Invest 99(1):19-23, 1997. 3. Buhler H, et al. Mol Cancer Res3(7):365-71, 2005.
Additional info:	Recognizes human Cytokeratin 18.

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Cytokeratin 18

Cat no: C9097-20B

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