Dectin-1 (C-type Lectin Domain Family 7 Member A, Dendritic Cell-associated C-type Lectin 1, DC-associated C-type Lectin 1, Beta-glucan Receptor, beta-GR, C-type Lectin Superfamily Member 12, CLEC7A, BGR, CLECSF12, DECTIN1, UNQ539/PRO1082) (PE)

Dectin-1 is predominantly expressed by cells of the monocyte/macrophage and neutrophil lineages, but also at lower levels by dendritic cells and a subpopulation of T cells. As a major leukocyte receptor for beta-glucan this molecule may have a key role in the immunomodulatory effects of beta-glucans and in the host response to fungal pathogens. Recent work has shown that Dectin-1 may stimulate reactive oxygen production in macropahges via the protein tyrosine kinase known as Syk.\n\nApplications:\nSuitable for use in Flow Cytometry. Other applications not tested.\n\nRecommended Dilutions:\nFlow Cytometry: Neat-1:10; 10ul labels 10e6 cells in 100ul.\nOptimal dilutions to be determined by the researcher.\n\nHybridoma:\nSpleen cells from immunized Fischer rats were fused with cells of the rat Y3 myeloma cell line\n\nRecommended Negative Control:\nI1904-84L1: IgG2b Negative Control (PE)\n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C. Reconstitute with sterile ddH2O. Aliquot and store at 4 degrees C. Reconstituted product is stable for 6 months as an undiluted liquid. Dilute only prior to immediate use. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.

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SPECIFICATIONS

Catalog Number

D1876-48P6

Size

100Tests

Applications

FC

Hosts

Rat

Reactivities

Mouse

Form

Supplied as a lyophilized powder from PBS, pH 7.4, 5% sucrose, 1% BSA, 0.09% sodium azide. Reconstitute with 1ml sterile ddH2O. Labeled with R-Phycoerythrin (PE).

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2b

References

1. Brown, G.D., et al. J. Exp. Med. 196: 407-412 (2002). 2. Taylor, P.R., et al., J. Immunol. 169: 3876-3882 (2002). 3. Reid, D.M., et al., J. Leuk. Biol. 76: 86-94 (2004). 4. Underhill, D.M., et al., Blood 106: 2543-2550 (2005). 5. Lefevre, L., et al., 5(9): e12828 (2010). 6. Fei, M., et al., PNAS USA 108(13): 5360-5365 (2011). 7. Gazi, U., et al., J. Biol. Chem. 286: 7822-7829 (2011). 8. McDonald, J.U., et al., FASEB J. 25(6): 1972-1982 (2011). 9. Dewals, B.G., et al., PLoS Negl. Trop. Dis. 4: e689 (2010). 10. Gal

Additional Info

Recognizes mouse Dectin-1. This antibody inhibits the binding of zymosan to macrophages via the beta-glucan receptor.