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DISC1 (Disrupted in Schizophrenia 1, RP4-730B13.1, KIAA0457)

Cat no: D8066-83K

DISC1 (Disrupted in Schizophrenia 1, RP4-730B13.1, KIAA0457)

Disrupted in schizophrenia 1 (DISC1) is a 100kD cytoplasmic scaffold protein that is associated with the development of schizophrenia, bipolar disorder, and recurrent major depression. It plays a role in post-synaptic density development and neurogenesis as well as centrosome and microtubule dynamics. DISC1 interacts with a range of intracellular proteins including Kinesin 1, NDE1, PDE4, GSK-3 beta, GRB2, PACAP, RAC1, TNIK, and FEZ1. It contains four coiled-coil domains aa366-394, aa452-505, aa602-666, and aa802-830 which mediate the assembly of DISC1 into a variety of multimers. DISC1 multimerization and its ability to interact with various binding parters are regulated by post-translational modifications and proteolysis. Alternate splicing generates additional isoforms of human DISC1 that are truncated before the first or following the third coiled coil domain. Within aa101-260, human DISC1 shares ~43% aa sequence identity with mouse and rat DISC1.\n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 2ug/ml\nImmunohistochemistry: 8-25ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

D8066-83K

Size

100ug

Applications

IHC, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.5mg/ml.

P Type

Mab

Purity

Purified by Protein A/G affinity chromatography.

Isotype

IgG1

Additional Info

Recognizes human DISC1.

Read more on Supplier website

Applications

IHC

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

More info

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Dro/Arth

More info

Applications

IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Dro/Arth, NHP, Yeast/Fun

More info
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