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DNA Ligase 1 (DNL1, LIG1)

DNA Ligase 1 (DNL1, LIG1)

Cat no: D3925-01K

Supplier: United States Biological
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Eukaryotic DNA ligases are ATP-dependent enzymes that catalyze the joining of single and double stranded DNA breaks, which is an essential step in DNA repair, replication and recombination. There are at least 4 DNA ligases, termed DNA ligase I-IV, have been cloned and characterized. DNA ligase-I is functionally homologous to the DNA ligase encoded by the S. cervisiae CDC9 gene. The joining of Okazaki fragments during lagging strand DNA replication is due to DNA ligase-I. DNA ligase-I (919 aa, chromosome 19q13.2-q13.3; mouse 916 aa, rat 919 aa) is a nuclear protein. Its deficiency causes immunodeficiency and cellular hypersensitivity of DNA-damaging agents. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:16,000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: D3925-01K
Reactivities: Human
Hosts: Goat
Applications: ELISA, Western Blot
Size: 100ug
Form: Supplied as a liquid in Tris saline, 0.02% sodium azide, pH7.3, 0.5% BSA.
P type: Pab
Isotype: IgG
Purity: Purified by affinity chromatography.
References: 1. Ferrari G, Rossi R, Arosio D, Vindigni A, Biamonti G, Montecucco A, Cell cycle-dependent phosphorylation of human DNA ligase I at the cyclin-dependent kinase sites. J. Biol. Chem. 2003 Sep 278 (39): 37761-7.
Additional info: Recognizes human LIG1/DNA Ligase 1. Sequence homology: rat

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