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DnaJ (HSP40)

Cat no: D4015-10

DnaJ (HSP40)

DnaJ is a basic 41kD Escherichia coli heat shock protein which belongs to the molecular chaperone class of proteins. The E. coli dnaJ gene is located in an operon with dnaK (1). The sequence of the dnaJ gene shares identity with eukaryotic cytosol and endoplasmic reticulum DnaJ homologues. These homologues are known to be involved in i) protein folding, and membrane translocation of newly synthesized proteins, and ii) initiation of translation. The N-terminal domain of the DnaJ protein constitutes the most highly conserved domain and it is used as the signature sequence of DnaJ family members. This so-called

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SPECIFICATIONS

Catalog Number

D4015-10

Size

25ug

Applications

WB

Form

Supplied as a liquid in 50mM HEPES, pH 7.4, 300mM KCl, 2mM DTT and 10% glycerol.

Purity

(same/more than) 90%; determined by SDS-PAGE analysis.

References

1. Zylicz, M., Yamamoto, T., McKittrick, N., Sell, S., Georgopoulos, C. (1985) J. Biol. Chem. 260: 7591-7598. 2. Deloche, et al. (1997) J. Biol. Chem. 272: 28539-28544.

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