Dock2 (Dedicator of Cytokinesis 2, AI662014, AW122239, CED-5, Dedicator of Cytokinesis Protein 2, Hch, MBC, Protein Hch)

Dock2 (Dedicator of cytokinesis 2), is a large protein involved in intracellular signaling networks and a member of the DOCK-A subfamily which function as activators of small G proteins. Dock2 specifically activates isoforms of the small G protein Rac and appears to be expressed specifically in leukocytes. These cells express both Rac1 and Rac2 and Dock2 has been shown to bind and promote nucleotide exchange on both of these isoforms. (Nishihara, 1999)\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.5mg/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nMouse spleen lysate \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

D8080-20B

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, 0.05% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Gotoh K., et al. (2008). Blood. 111(6):2973-76. 2. Nombela-Arrieta C., et al. (2007). J. Exp. Med. 204(3):497-510. 3. Nishihara H., et al. (1999). Biochem. Biophys. Acta. 1452(2):179-87.

Additional Info

Recognizes mouse DOCK2. Species sequence homology: human, horse, primates (93%). The sequence is 85.7% homologous in rat.