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Dopamine Transporter, CT (DAT)

Cat no: D9020-30A

Dopamine Transporter, CT (DAT)

DAT is principally expressed in the presynaptic membrane of dopaminergic nerve terminals, where it mediates the rapid removal of dopamine from the synaptic cleft after neuronal stimulation. As such, DAT plays an important role in regulating the availability of dopamine in the synaptic cleft and thus in modulating the physiological functions of dopamine.\nThe reuptake of dopamine by DAT is inhibited by psychostimulants such as cocaine and amphetamine.\n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry (frozen). Other applications not tested. \n\nRecommended Dilution: \nImmunohistochemistry: 1:1000\nWestern Blot: 1:1000,detects a band of approximately 88kD in human caudate cell lysates\nOptimal dilution determined by the researcher. \n\nRecommended Secondary Reagents: \nSheep anti rabbit IgG:HRP \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

D9020-30A

Size

100ul

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, NHP

Form

Supplied as a liquid in 10mM HEPES, pH 7.5, 0.1% BSA, 0.09% BSA and 50% glycerol.

P Type

Pab

Purity

Purified by affinity chromatography.

References

1. Mozley, L.H. et al. (2001). Striatal Dopamine Transporters and Cognitive Functioning in Healthy Men and Women. Am. J. Psychiatry 158: 1492 - 1499.

Additional Info

Specific for human dopamine transporter (DAT), also known as solute carrier family 6.\nSpecies Cross Reactivity: Monkey

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