Home  >  Products  >  Fen1 (FEN-1, Flap Structure-specific Endonuclease 1, MF1, Maturation Factor-1, RAD2, DNase IV)
Fen1 (FEN-1, Flap Structure-specific Endonuclease 1, MF1, Maturation Factor-1, RAD2, DNase IV)

Fen1 (FEN-1, Flap Structure-specific Endonuclease 1, MF1, Maturation Factor-1, RAD2, DNase IV)

Cat no: F0047-04

Supplier: United States Biological
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FEN-1 (Flap EndoNuclease and Five prime Exonuclease) is a 42kDa protein that functions as an endonuclease and cleaves branched DNA structures containing single-stranded 5' flaps or as an exonuclease on DNA nicks and gaps. FEN-1 endonuclease and exonuclease activities are stimulated by an association with proliferating cell nuclear antigen (PCNA) in the nucleus. FEN-1 is believed to have important roles in DNA replication and repair, and is required in cell-free DNA replication systems. Positive Control: HeLa, Raji and 3T3 cell lysates. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: F0047-04
Reactivities: Human, Mouse
Hosts: Rabbit
Applications: Western Blot
Size: 100ul
Form: As reported
P type: Pab
Purity: Whole antisera
Alternative names: Flap endonuclease 1
References: 1. Hiraoka, L.R., Harrington, J.J., Gerhard, D.S., Lieber, M.R. and Hsieh, C.L. (1995) Sequence of human FEN-1, a structure-specific endonuclease, and chromosomal localization of the gene (FEN1) in mouse and human. Genomics 25:220-5. 2. Warbrick, E., Coates, P.J. and Hall, P.A. (1998) Fen1 expression: a novel marker for cell proliferation. J Pathol 186:319-24. 3. Kim, K., Biade, S. and Matsumoto,Y. (1998) Involvement of flap endonuclease 1 in base excision DNA repair. J Biol Chem 273:8842-8. 4. Rumbaugh, J.A., Henricksen, L.A., DeMott, M.S. and Bambara, R.A. (1999) Cleavage of substrates with mismatched nucleotides by flap endonuclease-1. Implications for mammalian Okazaki fragment processing. J Biol Chem 274:14602-8. 5. Kolodner, R.D. and Marsischky, G.T. (1999) Eukaryotic DNA mismatch repair. Current Opinion Genet Dev 9:89-96.
Additional info: Recognizes human FEN-1. Species Crossreactivity: mouse

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