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FOXO3A (Forkhead Box O3A, Forkhead in Rhabdomyosarcoma-like 1, AF6q21 Protein, DKFZp781A0677, FKHRL1, FKHRL1P2, MGC12739, MGC31925)

Cat no: F9049-31L

FOXO3A (Forkhead Box O3A, Forkhead in Rhabdomyosarcoma-like 1, AF6q21 Protein, DKFZp781A0677, FKHRL1, FKHRL1P2, MGC12739, MGC31925)

Forkhead box O3A (FOXO3A) is a member of the forkhead family of transcription factors (FOXO), which play an important role in cellular proliferation, metabolism, stress tolerance, and aging. FOXO transcription factors have a characteristic forkhead domain and are located in the cytoplasm. Upon oxidative stress, FOXO3A translocates to the nucleus where binding to the DNA sequence 5'-[AG]TAAA[TC]A-3' occurs and IGF-1 is dephosphlorated, resulting in apoptosis. In order for the cell to survive, IGF-1 must be phosphlorated by AKT1/PKB at Thr32 and Ser-253, which will result in retention and inactivation of FOXO3A in the cytoplasm. Translocation of FOXO3A with the MLL gene has been associated with secondary acute leukemia.\n\nApplications:\nSuitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:20,000\nWestern Blot: 1:500-1:1,000 \nImmunohistochemistry: 1:50-1:100 \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

F9049-31L

Size

50ug

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

Additional Info

Recognizes human FOXO3A. Species Cross-Reactivity: mouse, rat.

Read more on Supplier website

Applications

IHC

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

More info

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Dro/Arth

More info

Applications

IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Dro/Arth, NHP, Yeast/Fun

More info
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