Fractalkine (Neurotactin)

Fractalkine, also known as neurotactin, is a membrane-bound CX3C chemokine. The mature protein is part of a 397-amino acid precursor and consists of a chemokine domain of 76 amino acids, a mucin stalk of 241 amino acids, a putative transmembrane domain of 18 amino acids, and an intracellular tail of 37 amino acids. Within the chemokine domain the first two cysteine residues are separated by 3 amino acids (CX3C). Fractalkine message is found in high abundance in the brain, kidney, lung and heart tissues. Fractalkine is chemotactic for monocytes and other leukocytes including NK cells, lymphocytes, and may play a role in brain inflammation. \n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:2000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

F6070-26

Size

200ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a lyophilized powder in 0.1% sodium azide.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

References

1. Bazan, J.F. et al. (1997) A new class of membrane-bound chemokine with a CX3C motif. Nature 385:640-644 2. Pan, Y. et al. (1997) Neurotactin, a membrane-anchored chemokine upregulated in brain inflammation. Nature 387:611-617 3. Imai, T. et al. (1997) Identification and molecular characterization of fractalkine receptor CX3CR1, which mediates both leukocyte migration and adhesion. Cell 91:521-530.

Additional Info

Reacts with recombinant and natural human fractalkine.