GCSc-(gamma) (E315) pAb

The GCLC gene consists of 16 exons and encodes the 636 amino acid protein (gamma)-GCSc ((gamma)-glutamylcysteine synthetase heavy subunit), also designated (gamma)-Lglutamate- L-cysteine ligase catalytic subunit (GLCLC). (gamma)-GCSc is expressed in hemocytes, brain, liver and kidney. (gamma)-GCSc associates with a regulatory or modifier subunit, (gamma)-GCSm ((gamma)-glutamylcysteine synthetase light subunit), to form a heterodimer, (gamma)-GCS. (gamma)-GCS is the first enzyme involved and the rate determining step in glutathione iosynthesis. Oxidants, cadium and methyl mercury upregulate the transcription of (gamma)-GCS. H2O2 regulation depends on the Yap1 protein and the presence of glutamate, glutamine and lysine. Cadium regulates transcription through proteins Met-4, Met-31 and Met-32. Cbf1, a DNA binding protein, inhibits transcription of (gamma)-GCS.

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SPECIFICATIONS

Catalog Number

BS2926

Size

100ug/100ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

liquid

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Antigen

Synthetic peptide, corresponding to amino acids 300-350 of Human GCSc-(gamma).

Species

GCSc-(gamma) (E315) pAb detects endogenous levels of GCSc-(gamma) protein.

Dilutions

WB: 1:500~1:1000

Swiss Prot

P48506

Storage Temp

Store at 4 degrees C short term. Aliquot and store at -20 degrees C long term. Avoid freeze-thaw cycles.

Concentration

1ug/ul