Gamma-glutamylcysteine synthetase (gamma -GCS) is the rate limiting enzyme for glutathione (L-gamma -glutamyl-L-cysteinylglycine, GSH) synthesis. GSH is ubiquitous in mammalian cells as a vital intra- and extracellular protective antioxidant. gamma -GCS is a heterodimer of a heavy catalytic subunit and a light regulatory subunit that is responsive to inflammation, phenolic ntioxidants, heat shock, oxidants and cytokines. The human gamma -GCS gene encoding the 367 amino acid catalytic subunit maps to chromosome 6p12. The human gamma -GCS gene encoding the regulatory subunit maps to chromosome 1p22-p21. The two subunits of gamma -GCS form a heterodimeric zinc metalloprotein that gains activity through formation of a reversible disulfide bond.