hnRNP A2B1 (hnRNPA2, hnRNPB1, Heterogeneous Nuclear Ribonucleoprotein A2B1)

RNA polymerase II transcripts in the nucleus are in complex with several different proteins called heterogeneous nuclear ribonucleoproteins (hnRNPs). These proteins act in biological activities such as transcription, pre-mRNA processing, cytoplasmic mRNA ranslation, and turnover. hnRNPs can be isolated either by immunoprecipitation or by sucrose gradient fractionation of cell extracts. Isolated hnRNPs consist of protein groups named A to U and many of these protein groups consist of more than one isoform. The major steadystate proteins of the isolated hnRNP complex are the A1, A2, B1, B2, C1, and C2 with a range of molecular weights (34-43kD). hnRNP-A2/B1 proteins are located in the nucleus of most tissues cells; however, the hnRNP-A2 protein is found also in the cytoplasm of skin and esophagus cells. In rat brain, hnRNP-A2/B1 proteins are found in neurons in the cerebral cortices, hippocampal formation, olfactory regions, caudate-putamin, and the supraoptic ucleus of the hypothalamus. Expression of hnRNP-A2/B1 proteins was found also in lung development and its over expression correlated with lung cancer.\n\nApplications: \nSuitable for use in ELISA, Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1-2ug/ml detects a band at 36kD.\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \n293T cell line\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

H6282-20A

Size

100ul

Applications

ELISA, ICC, IP, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Bov, Can, Ch/Bird, NHP

Form

Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.

P Type

Mab

Purity

Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.

References

1. Kamma, H., et al., Exp. Cell Res., 246, 399-411 (1999).2. Krecic, A.M., et al., Curr. Opin. Cell Biol., 11, 363-371 (1999). 3. Choi, Y.D., et al., Proc. Natl. Acad. Sci. USA, 81, 7471-7475 (1984). 4. Mizukami, K., et al., NeuroReport, 11, 3099-3102 (2000). 5. Montuenga, L.M., et al., Am. J. Respir. Cell Mol. Biol., 19, 554-562 (1998).

Additional Info

Recognizes human hnRNP A2B1 at ~36kD. Species Crossreactivity: monkey, bovine, canine, rat, mouse and chicken