hnRNP Q/R (Heterogeneous Nuclear Ribonucleoprotein Q, hnRNP Q, hnRNP-Q, HNRPQ, HNRPQ1, Heterogeneous Nuclear Ribonucleoprotein R, hnRNP R, HNRNPR, hnRNP-R, HNRPR, Glycine- and Tyrosine-rich RNA-binding Protein, GRYRBP, GRY-RBP, NS1-associated Protein 1, N

Heterogeneous nuclear ribonucleoprotein Q and R belong to a family of hnRNP proteins that are involved in RNA binding, RNA biosynthesis and mRNA transport from nucleus to cytoplasm (1-3). These two proteins are encoded by different gene but have 83% homology. hnRNP\nQ has three alternative splice variants (hnRNP Q1-3) (1-3). Methylation of carboxy-terminal arginine residues is required for nuclear localization (4). hnRNP Q binds to AUrich mRNA in conjunction with AUF1 and regulates mRNA decay (5). hnRNP Q isoforms play a crucial role in mediating nuclear function of survival of motor neuron (SMN) complex (6,7) and modulate RNA biosynthesis and hepatitis C virus replication (8). hnRNP R was identified recently and its function is still under investigation (9), however hnRNP R does not duplicate the biological function of hnRNP Q. Both hnRNP Q and R are present in cytoplasmic mRNP granules containing untranslated mRNAs (10) and both interact with SMN (11).\n\nApplications: \nSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4 degrees C with gentle shaking, overnight.\nImmunoprecipitation: 1:50\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

H6285-68

Size

100ul

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

(1) Dreyfuss, G. et al. (1993) Annu Rev Biochem 62, 289-321. (2) Burd, C.G. and Dreyfuss, G. (1994) Science 265, 615-21. (3) Krecic, A.M. and Swanson, M.S. (1999) Curr Opin Cell Biol 11, 363-71.\n(4) Passos, D.O. et al. (2006) Biochem Biophys Res Commun 346, 517-25. (5) Moraes, K.C. et al. (2003) Biol Chem 384, 25-37. (6) Mourelatos, Z. et al. (2001) EMBO J 20, 5443-52. (7) Rossoll, W. et al. (2002) Hum Mol Genet 11, 93-105. (8) Liu, H.M. et al. (2009) Virology 386, 249-56. (9) Hassfeld, W. et al. (1998) Nucleic Acids Res 26, 439-45. (10) Carty, S.M. and Greenleaf, A.L. (2002) Mol Cell Proteomics 1, 598-610. (11) Mourelatos, Z. et al. (2001) EMBO J 20, 5443-52.

Additional Info

Recognizes endogenous levels of total hnRNP Q and hnRNP R.

Alternative Names

Synaptotagmin Binding, Cytoplasmic RNA Interacting Protein, dJ3J17.2, FLJ25714, FLJ31626