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Insulin-Like Growth Factor I Receptor, Recombinant, Human, GST-Tag (IGF1R)

Cat no: I7661-20J

Insulin-Like Growth Factor I Receptor, Recombinant, Human, GST-Tag (IGF1R)

Human IGF1R (GenBank Accession No. NP_000875). GST fusion protein containing the Mr 46 kD cytoplasmic domain of the b-subunit of the human Insulin-like Growth Factor-1 Receptor Kinase, residues 930-1337, expressed in a baculovirus expression system. \n\nSpecific Activity: \n~100 nmol/min/mg at 25 degrees C using poly(Glu:Tyr) 4:1 substrate. Reaction conditions are 50mM Tris, pH 7.5, 5mM MgCl2, 5mM MnCl2, 1mM DTT, 250uM ATP. The kinase concentration was 0.5-1uM. \n\nApplications: \nEnzyme kinetics, autophosphorylation, and phosphorylation of target substrates.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and store at -70 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.

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SPECIFICATIONS

Catalog Number

I7661-20J

Size

10ug

Form

Supplied as a liquid in 50mM Tris, pH 7.5 and 1mM DTT.

Purity

(same/more than) 80% by SDS-PAGE

References

1. K. Baer, et al. Biochemistry 2001 40 14268\n2. N. Tennagels, et al. Biochem. Biophys. Res. Com. 1999 260 7

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