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Insulin Receptor, beta, Recombinant, Human, GST-Tag (IRb)

Cat no: I7661-26K

Insulin Receptor, beta, Recombinant, Human, GST-Tag (IRb)

Human INSR, Insulin receptor (beta) (GenBank Accession No. NM_000208). GST fusion protein containing the Mr 48kD cytoplasmic domain of the b-subunit of the human insulin receptor, residues 941-1343, expressed in a baculovirus expression system. \n\nSpecific Activity: \n~117 nmol/min/mg @ 25 degrees C using poly(Glu:Tyr) 4:1 substrate. Reaction conditions are 50mM Tris, pH 7.5, 30mM MgCl2, 1 mM DTT, 1.0mM ATP and 1mg/ml substrate. \n\nApplications: \nSuitable for use in Enzyme kinetics, autophosphorylation, and phosphorylation of target substrates. Other applications not tested. \n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and store at -70 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.

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SPECIFICATIONS

Catalog Number

I7661-26K

Size

10ug

Form

Supplied as a liquid in 50mM Tris, pH 7.5 and 1mM DTT.

Purity

(same/more than) 80% by SDS-PAGE

References

1. H. Al-Hasani et al. FEBS Lett. 1994 349 17

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