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Integrin, beta1 (CD29, Very Late Antigen, VLAb1, Platelet gplla)

Cat no: I7661-39A3

Integrin, beta1 (CD29, Very Late Antigen, VLAb1, Platelet gplla)

Human CD29 is the beta subunit of an integrin family of molecules expressed on diverse cell types which function as the major receptors for extracellular matrix and as cell-cell adhesion molecules. \n\nApplications: \nSuitable for use in Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nImmunohistochemistry: Fixed paraffin sections\nWestern Blot: 1-2ug/ml under non-reducing conditions\nFlow Cytometry: 10ug/ml\nOptimal dilutions to be determined by the researcher.\n\nFlow Cytometry Protocol:\n1. Five x 10e5 HPB-MLT cells were washed and incubated 45 minutes on ice with 80ul of I7661-39A3 at a concentration of 10ug/ml. \n2. Cells were washed twice and incubated with secondary reagent Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS.\n3. Cells stained positive with a mean shift of 1.3 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

I7661-39A3

Size

100ug

Applications

FC, IHC, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2, 0.5mg/ml gentamicin sulfate.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG1,k

References

1. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 1612-1613.

Additional Info

Recognizes the human CD29 integrin subunit. CD29 can form heterodimer pairs with at least nine different alpha subunits.

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