Integrin, beta5

Integrin beta5 is a 100-110kD type I transmembrane glycoprotein that forms a non-disulfide-linked heterodimer with Integrin aV. Integrin aVb5 is a receptor for vitronectin that is expressed on a wide variety of cell types including keratinocytes, fibroblasts, adhesive monocytes, and select endothelium. The 696 amino acid residue Integrin b5 extracellular region contains a vWF-A domain and a cysteine-rich tandem repeat region.\n\nApplications:\nSuitable for use in Western Blot, Flow Cytometry, Immuncytochemistry and ELISA. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 0.1-0.2ug/ml. The detection limit is ~5ng/lane under non-reducing and reducing conditions. \nFlow Cytometry: 50ug/ml, 10ul labels 1-2.5x10e5 MG-63 human osteosarcoma cells in a total reaction volume <200ul. The binding of unlabeled antibodies may be visualized by adding 10ul of a 25ug/ml stock solution of a secondary developing reagent such as anti-sheep lgG conjugated to a fluorochrome. \nImmunocytochemistry: 10ug/ml detects Integrin, beta5 in MG-63 human osteosarcoma cells. Cells were fixed with PBS, 4% paraformaldehyde for 20 minutes at RT and blocked with PBS, 10% normal donkey serum, 0.1% Triton X-100, 1% BSA for 45 minutes at RT. After blocking, cells were incubated with diluted I7661-43E overnight at 4 degrees C followed by Rhodamine Red coupled anti-sheep IgG at RT in the dark for one hour. Between each step, cells were washed with PBS, 0.1% BSA. \nDirect ELISA: 0.5-1.0ug/ml. The detection limit is ~0.2ng/well. \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.пїЅ

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SPECIFICATIONS

Catalog Number

I7661-43E

Size

100ug

Applications

ELISA, FC, ICC, WB

Hosts

Sheep

Reactivities

Hum

Form

Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.

P Type

Pab

Purity

Purified by affinity chromatography.

Isotype

IgG

Additional Info

Recognizes human Integrin beta5. Shows <5% crossreactivity with recombinant human Integrin beta1, beta3, beta4, beta6 and recombinant mouse Integrin beta7. Species Sequence Homology: mouse; 91%, canine; 89%