Interferon gamma, Recombinant, Mouse (CHO-Derived)

This glycosylated interferon is constitutively produced by Chinese Hamster Ovary (CHO) cells. The CHO cells have been transformed by a plasmid containing mouse coding sequences for gamma interferon as well as for dihydrofolate reductase. Biochemical analysis of CHO-derived mouse gamma interferon and gamma interferon as produced by mouse splenocytes revealed no structural differences. The major advantage of CHO-cell derived hi-pure recombinant mouse gamma interferon over natural mouse gamma interferon is the absence of contaminating lymphokines. This ensures that the effects observed in experimental results can be attributed exclusively to the hi-pure mouse gamma interferon used. \n\nOne unit of Mouse Gamma Interferon approximates the bioactivity contained in one unit of the mouse gamma interferon standard prepared by NIH (Gg02-901-533). \n \nSource:\nMouse, CHO-derived \n \nRecommended Dilution:\nUse protein stabilized phosphate buffered saline, pH 8. (Do not use fresh serum).\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at-20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with 1ml distilled or de-ionized water. Aliquot and store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

I7662-18Z

Size

10KU

Form

Supplied as a lyophilized powder in PBS, 10% mouse albumin, 0.01% gelatin. Reconstitute the vial by injection of 1 ml distilled or de-ionized water (Caution: vial is under vacuum).

Purity

Purified

References

1. Dijkmans, R et al; Molecular cloning of murine interferon gamma (MuIFN-gamma) cDNA and its expression in heterologous mammalian cells. J Interferon Res 1985, 5: 511