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Interleukin 1 alpha (IL-1a)

Cat no: I7663-02E

Interleukin 1 alpha (IL-1a)

IL-1alpha is a non-secreted proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1alpha and IL-1beta binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1beta is a secreted cytokine, IL-1alpha is predominantly a cell-associated cytokine.\n\nRecommended Secondary Reagents: \nSheep anti Rabbit IgG (Aff. Pur.): HRP\n\nApplications:\nSuitable for use in ELISA, Western Blot, Neutralization. Other applications not tested.\n\nRecommended Dilution:\nELISA: 0.5ug/ml\nWestern Blot: 0.1-0.2ug/ml\nNeutralization: 0.03-0.05ug/ml to yield one half maximal inhibition (ND50) of mouse IL-1 alpha\nOptimal working dilutions to be determined by researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

I7663-02E

Size

100ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Mouse

Form

Supplied as a lyophilized powder in PBS. No preservatives added. Reconstitute with 0.1ml distilled water.

P Type

Pab

Purity

Purified by affinity chromatography.

Isotype

IgG

Additional Info

Specific for mouse interleukin-1 alpha (IL-1 alpha).

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