Interleukin 13 (IL-13)

Interleukin-13 (IL-13) is a multifunctional cytokine whose net principle action is to diminish inflammatory responses. Interleukin (IL)-13 is an important cytokine secreted from type 2 helper T lymphocytes. It is essential for modulating IgE synthesis by human B cells. Previous studies showed that polymorphisms in the IL-13 gene were associated with serum total IgE or allergic asthma. Interleukin (IL)-13 is a pleiotropic cytokine, which shares many biological functions with IL-4. The receptor subunits of IL-13 consist of IL-4Ralpha, IL-13Ralpha1 and IL-13Ralpha2.\n\nApplications: \nSuitable for use in Flow Cytometry, ELISA, Western Blot and Dot Blot. Other applications not tested.\n\nRecommended Dilutions:\nFlow Cytometry: 0.1-1ug per 10e6 cells for detection of intracellular IL-13 in PMA/ionophore-stimulated rat splenocytes. \nWestern Blot: 1:500-1:1000 \nDot Blot: 1:500-1:1000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

I8435

Size

500ug

Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Rat

Form

Supplied as a liquid in PBS, pH 7.2. Carrier and preservative free.

P Type

Mab

Purity

Purified by Protein G affinity chromatography. ((same/more than)95% by SDS-PAGE).

Isotype

IgG1

References

1. Prussin, C. and D. Metcalfe (1995) Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J. Immunol. Meth. 188:117-128. 2. Takizawa, F. et al. (1993) Binding of phycoerythrin and its conjugates to murine low affinity receptors for immunoglobulin G. J. Immunol. Meth. 162:269-272. 3. Sander, B. et al. (1993) Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. J. Immunol. Meth. 166:201-214. 4. Bj

Additional Info

Recognizes recombinant and native rat IL-13.