Interleukin 3 (IL-3) (Biotin)

Applications: \nSuitable for use in Immunofluorescence, Flow Cytometry and ELISA. Other applications not tested.\n\nRecommended Dilution:\nImmunofluorescence: Useful for intracytoplasmic staining. Recombinant human IL-3, purified antibody (I7663-39L or I7663-39P) should be used as a specificity control.\nFlow Cytometry: Recombinant human IL-3, I7663-39N or IgG1, Rat Isotype Control (Biotin)I1904-76U2 should be used as a specificity control.\nELISA: Preferred detection antibody in a sandwich ELISA for quantifying human IL-3 protein levels. I7663-39P should be paired with I7663-39L as the capture antibody, with purified recombinant human IL-3 as the standard.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

I7663-39P

Size

500ug

Applications

ELISA, FC, IF

Hosts

Rat

Reactivities

Hum

Conjugates

Biotin

Form

Supplied as a liquid in PBS, 0.1% sodium azide. Labeled with Biotin.

P Type

Mab

Purity

Purified

Isotype

IgG1,k

References

1. Abrams, J.S., M.-G. Roncarolo, H.. Yssel, U. Andersson, G.J. Gleich, and J.E., Silver. 1992. Strategies of anti-cytokine monoclonal antibody development: Immunoassay of IL-10 and IL-5 in clinical samples. Immunol. Rev. 127:5-24.\n2. Abrams, J., J. Silver, R. van Dyke, and G. Gleich. 1994. Eosinophil-active cytokines in human disease: Development and use of monoclonal antibodies to IL-3-, IL-5, GM-CSF. In Eosinophils in Allergy and Inflammation (A. Kay and G. Gleich, eds). Marcel Dekker, New York, pp. 133-157.\n3. Abrams, J. 1995. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. In Current Protocols in Immunology (J. Coligamn, A. Kruisbeek, D. Margulies, E. Shevach, W. Strober, eds.). John Wiley and Sons, New York. Unit 6.20.\n4. Kaushansky, K. 1992. The structure-function relationships of interleukin-3: An analysis based on the function and binding characteristics of a series of interspecies chimera of gibbon and murine IL-3. J. Clin. Invest. 90:1879-1888.

Additional Info

Recognizes human Interleukin-3 (IL-3).