Interleukin 33 (IL33, Interleukin-33, IL-33, Interleukin-1 Family Member 11, IL-1F11, Nuclear Factor from High Endothelial Venules, NF-HEV, C9orf26, IL1F11, NFHEV)

Interleukin-33 (IL-33), also known as NF-HEV and DVS 27, is a member of IL-1 cytokine family. IL-33 is constitutively expressed in smooth muscle and airway epithelia and is upregulated by IL-1 Alpha or Beta in arterial smooth muscle, dermal fibroblasts, and keratinocytes. IL-33 is synthesized as a 270aa, 32kD proform that localizes to the nucleus and may regulate gene transcription. Cleavage generates mature IL-33 as an 18kD C-terminal proinflammatory fragment which binds IL-1 R4/ST2 on Th2 and mast cells. Mature human IL-33 shares 55% aa identity with mouse IL-33.\n\nApplications:\nSuitable for use in ELISA, Western Blot, Neutralization and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nELISA: To detect hIL-33 by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5-2ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-33 as a detection antibody, allows the detection of at least 0.2-0.4ng/well of recombinant hIL-33.\nWestern Blot: To detect hIL-33 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-33 is 1.5-3ng/lane, under either reducing or non-reducing conditions.\nImmunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human breast malignant ductal adenocarcinoma. The recommended concentration is 0.125ug/ml-0.25ug/ml with an overnight incubation at 4 degrees C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended.\nNeutralization: To yield one-half maximal inhibition of the biological activity of Human IL-33 (1ng/ml), a concentration of 0.2-0.4ug/ml of this antibody is required.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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