IRAK, CT (IL-1 Receptor-associated Kinase)

Nuclear factor kappa B (NF-kB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFa and LPS. A serine/threonine protein kinase associated with IL-1 receptor (IRAK) and its homologue mouse pelle-like protein kinase (mPLK) were identified recently (1,2). IRAK is associated with the IL-1 receptor subunits IL-1RI and IL-1RAcP after IL-1 binding and serves as a signaling molecule to mediate IL-1 response (3). IRAK mediates a signaling cascade leading to NF-kB activation by members in IL-1 family including IL-1 and a novel cytokine IL-18 (also termed IGIF) (1,4).\n\nApplications:\nSuitable for use in Western Blot and Immunoprecipitation. Other applications have not been tested.\n\nRecommended Dilution:\nWestern Blot: 1:500\nImmunoprecipitation: 2-4ug per sample\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nTHP-1 or HeLa cells can be used. 80kD band should be detected in the lysate from non-activated cells.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

I8600

Size

100ug

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, 0.02% sodium azide, before the addition of glycerol to 40%.

P Type

Pab

Purity

Purified IgG.

Isotype

IgG

References

1. Cao Z; Henzel WJ; Science 1996; 271:1128-31.2. Trofimova M; . J Bio Chem 1996; 271: 17609-12.3. Jianing Huang, Proc Natl Acad Sci USA 1997;94:12829-128324. Robinson D, Shibuya K, Mui A, Zonin F, Murphy E, Sana T, Hartley SB, Menon S, Kastelein R, Bazan F, O'Garra A. IGIF does not drive Th1 development but synergizes with IL-12 for interferon-gamma production and activates IRAK and NF-kB. Immunity 1997;7:571-581\n

Additional Info

Recognizes 80kD band should be detected in the lysate from non-activated cells. It has no crossreactivity to IRAK2.\nSpecies Crossreactivity: Human, mouse and rat.