IRAK, phosphorylated (Ser376) (IL-1 Receptor-associated Kinase) (FITC)

IRAK1 binds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Isoform 1 binds rapidly but is then degraded allowing isoform 2 to mediate a slower, more sustained response to the cytokine. Isoform 2 is inactive suggesting that the kinase activity of this enzyme is not required for IL-1 signaling. Once phosphorylated, IRAK1 recruits the adapter protein PELI1. This protein is partially responsible for IL1-induced upregulation of the transcription factor NF-kappa B.\n\nApplications: \nSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nELISA: 1:1000\nWestern Blot: 1:100-1:500\nImmunohistochemistry: 1:10-1:50\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze FITC conjugates. Light sensitive. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

I8600-07-FITC

Size

200ul

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2. Contains ~0.09% sodium azide. Conjugated to FITC.

P Type

Pab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG

References

1. De Nardo, D., et al., J. Biol. Chem. 280(11):9813-9822 (2005). 2. Siedlar, M., et al., Int. J. Cancer 114(1):144-152 (2005). 3. Huang, Y., et al., J. Biol. Chem. 279(49):51697-51703 (2004). 4. Noubir, S., et al., J. Biol. Chem. 279(24):25189-25195 (2004). 5. Cuschieri, J., et al., Shock 21(2):182-188 (2004).

Additional Info

Recognizes endogenous levels of human IRAK only when phosphorylated at serine 376.