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ISLR2 (Immunoglobulin Superfamily Containing Leucine-Rich Repeat 2, SST273)

Cat no: I8782-61

ISLR2 (Immunoglobulin Superfamily Containing Leucine-Rich Repeat 2, SST273)

ISLR2 (Ig-superfamily containing LRR protein 2; also SST273) is a 100-110kD member of the LRR family of proteins. It is expressed on developing motor, sensory and interneurons. Mouse ISLR2 is 745 amino acids (aa) in length. It is a type I transmembrane glycoprotein that contains a 571 aa extracellular domain (ECD) and a 132 aa cytoplasmic region. The ECD shows multiple LRRs (aa 21-231) and one Ig-like domain (aa 251-374). Mouse ISLR2 apparently has one alternate start site at Met130. Over aa 20-590, mouse ISLR2 is 89% and 98% aa identical to human and rat ISLR2, respectively.\n\nApplications: \nSuitable for use in Direct ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nDirect ELISA: 0.5-1ug/mL detection limit for rmISLR-2 ~ 0.8ng/well. \nWestern Blot: 0.1-0.2ug/mL detection limit for rmISLR-2 ~ 5ng/lane under non-reducing and reducing conditions.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

I8782-61

Size

100ug

Applications

ELISA, WB

Hosts

Sheep

Reactivities

Mouse

Form

Supplied as a lyophilized powder from PBS with 5% trehalose. Reconstitute with 200ul 40-50% glycerol, PBS.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

Additional Info

Recognizes mouse ISLR-2.

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SUPPLIER INFO

Applications

ELISA

Reactivities

Hum

More info

Applications

IF

Hosts

Mouse

More info

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info

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