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Itk (IL-2 Inducible T-cell Kinase, Emt, Tsk, T Cell Signaling Protein)

Cat no: I9200-05

Itk (IL-2 Inducible T-cell Kinase, Emt, Tsk, T Cell Signaling Protein)

Itk is a T cell signaling protein. The Tec family of non-receptor tyrosine kinases is composed of six proteins designated Tec, Itk (also known as Emt or Tsk), Btk (previously known as Atk, BPK or Emb), Bmx, Txk (also known as Rlk) and Dsrc28C. All members of the family contain SH3 and SH2 domains and, with the exception of Txk and Dsrc28C, also contain pleckstrin homology (PH) and Tec homology (TH) domains in their amino termini.\n\nApplications: \nSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 0.5-2ug/ml detects Itk in 20ug of a Jurkat cell RIPA lysate transferred to a PVDF membrane following SDS-PAGE. Note: Do not use nitrocellulose because the antibody was raised against immunogen bound to nitrocellulose.\nImmunoprecipitation: 4ug immunoprecipitated Itk from 500ug of Jurkat cell RIPA lysate.\nOptimal dilutions to be determined by the researcher.\n\nRecommended Secondary Antibody:\nI1904-39: IgG, X-Adsorbed (HRP) Pab Gt x Rb\nI1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt x Rb\nI1904-46Q: IgG, H&L (HRP) Pab Gt x Rb\n\nPositive Control Included: \nJ9000: Jurkat cell lysate. Add 2.5ul of 2-mercapto- ethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

I9200-05

Size

250ug

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide before the addition of glycerol to 30%.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

1. Gibson, et al., J. Immunol. 156: 2716 (1996). 2. Yamada, et al., Biochem. Biophys. Res. Commun. 192: 231 (1993).

Additional Info

Recognizes Itk, Mr ~72kD. Protein may appear as a doublet in some samples. Species Crossreactivity: human and mouse.

Read more on Supplier website

Applications

ELISA

Reactivities

Hum

More info

Applications

IF

Hosts

Mouse

More info

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info
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