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KBRAS2 (NF-kappa-B inhibitor-interacting Ras-like protein 2, I-kappa-B-interacting Ras-like protein 2, Kappa B-Ras protein 2, KappaB-Ras2, NKIRAS2)

Cat no: K0079-52A

KBRAS2 (NF-kappa-B inhibitor-interacting Ras-like protein 2, I-kappa-B-interacting Ras-like protein 2, Kappa B-Ras protein 2, KappaB-Ras2, NKIRAS2)

KappaB ras-1 and kB-ras-2 are two small proteins that similar to Ras-like small GTPases that associate with IkappaB (IkB), an inhibitor of the transcription factor NF-kB. IkB exists in two homologous forms, IkB-a and IkB-b, although IkB-b contains a unique 47aa region within its ankyrin domain. While inactive IkB-a-NF-kB complexes can shuttle in and out of the nucleus, IkB-b-NF-kB complexes are retained exclusively in the cytoplasm. It is suggested that kB-ras proteins preferentially bind to the IkB-b form through this unique insert within the ankyrin region, thus modulating the cellular location of IkB-b and regulating the rate of degradation of IkB-b. This antibody is specific for kB-ras2 and has no cross-reactivity to kBras1.\n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.5-1ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nRAW264.7 cell lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

K0079-52A

Size

50ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified

Isotype

IgG

References

1. Fenwick C, et al (2000) Science. 287:869-73. 2. Chen Y, et al (2003) J. Biol. Chem. 278:23101-6.

Additional Info

Recognizes human KappaB ras2. Species Crossreactivity: mouse and rat.

Read more on Supplier website

Applications

ELISA

Reactivities

Hum

More info

Applications

IF

Hosts

Mouse

More info

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info

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