KCC2 (K, Cl Cotransporter 2)

Chloride is a critical component of all living cells. It is also the single most dominant diffusible anion inside of most cells-the others are mostly impermeable organic anions. Since cytoplasmic electroneutrality is maintained under normal physiological environment, changes in cellular chloride level is accompanied by total cell solute content. The cation chloride cotransporters (CCC) protein family is involved in the electroneutral movement of ions across the cell membrane.\n\nThe K-Cl cotransporters mediate the coupled movement of K+ and Cl- ions across the cell membranes. This transport process is involved in the regulatory volume decrease in response to cell swelling in red cells and vectorial movement of Cl- ions across kidney epithelia. The net direction of this transport is out of the cells. At least four isoforms of KCC have been identified (KCC1, KCC2, KCC3 and KCC4) and functionally characterized. KCC are predicted to have the same protein structure: 12 transmembrane domains with a large extracellular loop with potential N-glycosylation sites, and the cytoplasmic N- and C-termini.\n\nApplications: \nSuitable for use in Western Blot and ELISA. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-10ug/ml using Chemiluminescence technique.\nELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well.\nOptimal dilutions to be determined by the researcher.\n\nControl Peptide:\nK0120: KCC2, Rat, Control Peptide\n\nStorage and Stability:\nFor long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

K0120-06

Size

50ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Mouse, Rat

Form

Supplied as liquid in 100mM Tris, pH 7.5, 0.2% BSA, 0.05% sodium azide, 40% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Payne, J.A., et al., J. Biol. Chem. 271: 16,245 (1996). 2. Gillen, C.M., et al., J. Biol. Chem. 271: 16,237 (1996). 3. Mount, D.B., et al., J. Exptl. Biol. 201: 2091 (1998). 4. O'Neill, W.C., Am. J. Physiol. 276: C995 (1999).

Additional Info

Recognizes rat KCC2. No significant sequence homology is seen with other KCCs or CCC. Species Sequence Homology: Human and mouse - 94%.