Keratin 2e (Keratin Type II Cytoskeletal 2 Epidermal, Keratin-2 Epidermis, Epithelial Keratin-2e, Keratin-2e, K2e, KRT2E, Keratin 2, KRT2, KRT2A, Cytokeratin-2e, CK-2e, MGC116967, MGC116968, Type-II Keratin Kb2)

Keratin 2e represents an excellent marker to study terminal epidermal differentiation. \n\nApplications: \nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry (frozen, paraffin): 1:20 PAP/ABC. \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

K0199-04E

Size

1ml

Applications

IHC, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Bov

Form

Supplied as a liquid.

P Type

Mab

Purity

Supernatant

Isotype

IgG1

References

1. Collin C., et al., Exp. Cell Res. 202: 132-141.

Additional Info

Recognizes epidermal cells in uppermost suprabasal layers including scalp, foot and sole. Recognizes individual cells within epidermis of tongue and mamille (co-localization with cytokeratin 10). Reactive on hyperkeratosis of diverse viral and genetic origin. The antibody does not react with palate cytokeratin 2p. Species Crossreactivity: bovine (calf hoof epidermis, snooze epithelium; negative with tongue epithelium), mouse/rat (heterogeneously on foot sole epidermis only; negative with epidermis of other body sites).