Kir2.3 (IRK3, BIR11, Kcnj4)

Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient (1). They are present in the membranes that surround all biological cells because their main function is to regulate the flow of ions across this membrane. Whereas some ion channels permit the passage of ions based on charge,others conduct based on a ionic species, such as sodium or potassium. Furthermore, in some ion channels, the passage is governed by a gate which is controlled by chemical or electrical signals, temperature, or \nmechanical forces. There are a few main classifications of gated ion channels. There are voltage- gated ion channels, ligand-gated, other gating systems and finally those that are classified differently, having more exotic characteristics.\n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1ug/ml if results are poor, use lysate without boiling, heat at 37C for 15 min.\nImmunohistochemistry: 0.1-1ug/ml (HRP), 1-10ug/ml (FITC)\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

K1859

Size

100ug

Applications

IHC, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, pH7.4, 0.09% sodium azide, 50% glycerol.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Hille B. (2001) Ion Channels of Excitable Membranes, 3rd Ed., Sinauer Associated Inc.: Sunderland, MA USA. 2. www.iochannels.org 3. Zobel C., et al. (2003) J Physiol. 550: 365-372. 4. Panama B.K., McLerie M., and Lopatin A.N. (2007) Am J Physiol Heart Circ Physiol. 293: H3558-H3567. 5. Munoz V., et al. (2007) Heart Rhythm. 4(4): 487-496. \n

Additional Info

Recognizes Kir2.3 at ~45kD. Species Crossreactivity: human, rat, mouse. Does not crossreact with Kir2.1 or Kir2.2.