KLF8 (Basic krueppel-like factor 3, Zinc finger protein 741)

KLF8 (Krueppel-like factor 8) is a protein that in humans is encoded by the KLF8 gene. It is also named as ZNF741, or BKLF3. Kruppel-like factor proteins, including KLF8, all contain a characteristic C-terminal DNA-binding domain containing 3 Kruppel-like zinc fingers, and they all recognize CACCC and GC boxes present in promoters and enhancers. The International Radiation Hybrid Mapping Consortium mapped the KLF8 gene to the X chromosome (WI-18614). Suske et al. (2005) stated that the human KLF8 gene maps to chromosome Xp11.21, and the mouse Klf8 gene to chromosome XF3. van Vliet et al. (2000) identified a cDNA encoding KLF8, also called ZNF741. Expression of KLF8 or only its zinc fingers showed that the fingers could bind to a CACCC box. Sequence analysis predicted that the 359-amino acid protein contains a nuclear localization signal upstream of the zinc fingers, a valine-rich hydrophobic stretch, and a pro-val-asp-leu-ser (PVDLS) motif. Yeast 2-hybrid and GST pull-down analysis demonstrated that CTBP2 interacts with KLF8 through the PVDLS motif.\n\nUniProt Number:\nO95600\n\nGene ID:\nKLF8\n\nApplications:\nSuitable for use in Western Blot.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Handling:\nStore at -20?C for one year. After reconstitution, store at 4?C for one month. Can also be aliquoted and stored frozen at -20?C for long term.\nAvoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

144812

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a lyophilized powder. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Lossi, A.-M., Laugier-Anfossi, F., Depetris, D., Gecz, J., Gedeon, A., Kooy, F., Schwartz, C., Mattei, M.-G., Croquette, M.-F., Villard, L.Abnormal expression of the KLF8 (ZNF741) gene in a female patient with an X;autosome translocation t(X;21)(p11.2;q22.3) and non-syndromic mental retardation.J. Med. Genet. 39: 113-117, 2002.\n2. Suske, G., Bruford, E., Philipsen, S.Mammalian SP/KLF transcription factors: bring in the family.Genomics 85: 551-556, 2005.\n3. van Vliet, J., Turner, J., Crossley, M.Human Kruppel-like factor 8: a CACCC-box binding protein that associates with CtBP and represses transcription.Nucleic Acids Res. 28: 1955-1962, 2000.\n

Additional Info

Recognizes human KLF8. No crossreactivity with other proteins.