KLHL15 (Kelch-like Protein 15, KIAA1677)

KLHL15 is a member of a growing superfamily of kelch repeat-containing proteins. The kelch motif was discovered in the sequence of the Drosophila kelch ORF1 protein and forms a conserved beta-propeller tertiary structure. These kelch-like proteins have been implicated in embryogenesis and carcinogenesis through cytoskeleton organization. KLHL15 mRNA is widely expressed in many tissues and is thought to be involved in protein ubiquination and cytoskeleton organization.\n\nApplications:\nSuitable for use in Immunofluorescence, ELISA, Western Blot and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-2ug/ml\nImmunofluorescence: 20ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nHeLa Cell Lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

146461

Size

100ug

Applications

ELISA, ICC, IF, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Yoshida K. Identification and characterization of a novel kelch-like gene KLHL15 in silico. Oncol. Rep. 2005; 13:1133-7. 2. Robinson DN and Cooley L. Drosophila kelch is an oligomeric ring canal actin organizer. J. Cell Biol. 1997; 138:799-810. 3. Adams J, Kelso R, and Cooley L. The kelch repeat superfamily of proteins: propellers of cell function. Trends Cell Biol. 2000; 10:17-24.

Additional Info

Recognizes human KLHL15. Species Crossreactivity: mouse and rat.