Klotho (KL)

Animal models of human aging, which display the characteristic diseases associated with aging, provide insight into the cause of such diseases. Recently a transgenic mouse model, called Klotho, with several premature aging phenotypes has been described. They display premature aging phenotypes such as Osteoporosis, age related skin changes, ectopic calcifications, atrophy of genital organs and thymus, emphysema and short life span (1). KL protein associated with the Klotho mutation is a 1014 amino acid long peptide. It has a putative signal sequence at its N-terminus and a single transmembrane domain near its C-terminus, which is postulated to anchor it to the membrane (2). The extracellular domain has two internal repeats, KL-1 and KL-2 which have 20-40% sequence identity to bacterial b-glucosidase of plants and animals and mammalian lactase glycosylceramidase suggesting a role for Klotho in sphingolipid metabolism (2). kl gene expression was observed to be tissue specific. Improvement of systemic aging phenotypes in kl/kl mice occurs even when the exogenous expression was limited to some organs, suggesting that KL associated aging is regulated through a humoral signaling pathway. KL has been reported to be localized on the cell surface when expressed on Cho cells (1). Human kl cDNA is expected to encode a protein of 1012 amino acids and has 86% homology to mouse kl. Alternative splicing produces two isoforms (a full length membrane bound and the secreted form).\n\nApplications:\nSuitable for use in Western Blot, ELISA, Immunoprecipitation and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-5ug/ml. Using Chemiluminescence technique.\nELISA: 1:10,000-1:100,000. Using 50-100 ng of control peptide/well.\nImmunohistochemistry: 2-20ug/ml.\nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

K1893-01L

Size

50ug

Applications

ELISA, IHC, IP, WB

Hosts

Rat

Reactivities

Mouse

Form

Supplied as a liquid in PBS, pH 7.2, 0.1% BSA, 0.05% sodium azide, 40% glycerol.

P Type

Mab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG2a

References

1. Segawa H, 2006, Am J Physiol Renal Physiol, Feb 2007; 292: F769- F779. -IHC. 2. Kurosu H. 2006, JBC, 281: 6120- 6123. - IP 3. Koro-o, M. Et al. (1997) Nature 390, 45-51. 4.

Additional Info

Recognizes mouse Klotho.