KLRA1, ID (Killer Cell Lectin-Like Receptor Subfamily A, Member 1, KLRA#, LY49L, Ly-49L, Ly49, MGC126520, MGC126522)

KLRA1 (also known as Ly49L) is a member of the LY49 family of receptors in Natural Killer (NK) cells that bind to major histocompatibility complex (MHC) class 1. These proteins are classified as either activating or inhibitory receptors based on whether they possess an immunoreceptor tyrosine-based inhibitory motif (ITIM) in their cytoplasmic region (for inhibitory receptors), or an immunoreceptor tyrosine-based activation motif (ITAM) that transmits activating signals resulting in phosphorylation of several substrates. KLRA1 is thought to be an activating receptor, inducing DAP12 phosphorylation in response to antibody-mediated cross-linking of KLRA1 on NK cells. At least three isoforms of KLRA1 are known to exist; this antibody recognizes only the long isoform.\n\nApplications:\nSuitable for use in Immunofluorescence, ELISA, Western Blot and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-2ug/ml\nImmunofluorescence: 20ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nMouse Spleen Tissue Lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

146377

Size

100ug

Applications

ELISA, ICC, IF, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Makrigiannis AP, Etzler J, Winkler-Pickett R, et al. Identification of the Ly49L protein: evidence for activating counterparts to inhibitory Ly49 proteins. J. Leuk. Biol. 2000; 68:765-71. 2. Smith HR, Karlhofer FM, and Yokoyama WM. Ly-49 multigene family expressed by IL-2-activated NK cells. J. Immunol. 1994; 153:1068-79. 3. Brennan J, Mager D, Jefferies W, et al. Expression of different members of the Ly-49 gene family defines distinct natural killer cell subsets and cell adhesion properties. J. Exp. Med. 1994; 180:2287-95.

Additional Info

Recognizes human KLRA1. Species Crossreactivity: mouse and rat.