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Leukocyte Immunoglobulin-like Receptor 6 (LIR6, LILRA1, CD85i)

Cat no: L2028-55

Leukocyte Immunoglobulin-like Receptor 6 (LIR6, LILRA1, CD85i)

Leukocyte Immunoglobulin-like Receptor 6 (LIR6), also called LILRA1 or CD85i, is a glycoprotein member of the LIR family of leukocyte Ig-like receptors. As an activating LIR, LIR-6 has a short cytoplasmic tail and a charged aa within the TM domain which interacts with FcRg. Both four (LIR-6a) and two (LIR-6b) Ig-like domain forms are expressed by monocytes and B cells. Human LIR6 has no non-primate ortholog, but shows up to 80% aa identity with human ILT-1c and KIR-p91/PIR-B. A mouse homolog, gp49B2, has structural similarity but limited sequence identity.\n\nApplications: \nSuitable for use in Western Blot and Direct ELISA. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1-2ug/ml. Using a colorimetric detection system, the detection limit for rhLIR6 is ~5ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. \nDirect ELISA: 0.5-1.0ug/ml. The detection limit for rhLIR6 is ~5ng/well. \nOptimal dilutions should be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n

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SPECIFICATIONS

Catalog Number

L2028-55

Size

100ug

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

Additional Info

Recognizes human LIR6 in Direct ELISAs and Western Blots. No crossreactivity with rhILT2, rhILT4, rhILT5, rhLIR4, rhLIR5 or rhLIR8.

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