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LIM Domain Kinase 2, phosphorylated (Thr505) (LIM Kinase 2, LIMK 2, LIMK2, LIMK-2)

Cat no: L2198-27D

LIM Domain Kinase 2, phosphorylated (Thr505) (LIM Kinase 2, LIMK 2, LIMK2, LIMK-2)

LIMK2 is a member of the family of LIM kinase (LIMK) which also includes LIMK1. The protein is a regulator of actin dynamics. LIMK2 controls the activity of cofilin, as it phosphorylates it on serine 3. The activity of the protein is regulated by the members of the Rho-GTPases family: Rho, Rac and Cdc42 via their respective effector molecules Rho-kinase (ROCK) and p21-activated kinases (PAK) PAK1 and PAK4. Overexpression of LIMK2 in a variety of cell lines results in increased actin polymerisation. Several LIMK2 mRNA isoforms were identified including LIMK2a, LIMK2b and tLIMK2. Human LIMK2 gene is mapped on chromosomal region 22q12.2.\n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:500-1:1000\nImmunohistochemistry (paraffin): 1:50-1:100\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nNIH/3T3 cells\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

L2198-27D

Size

50ug

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Karla, A. et al. Journal of Histochemistry and Cytochemistry. 54: 487-501 (2006).

Additional Info

Recognizes human LIMK2, phosphorylated at Thr505.

Read more on Supplier website

Applications

ELISA

Reactivities

Hum

More info

Applications

IF

Hosts

Mouse

More info

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info

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