Lin28b (Protein Lin-28 Homolog B, Lin-28B, Lin-28.2)

LIN28A and LIN28B are conserved, developmentally regulated RNA binding proteins that inhibit the processing and maturation of the let-7 family of miRNAs (1,2). The let-7 miRNAs have been implicated in repression of oncogenes such as ras, myc and Hmga2 (3). It has recently been shown that LIN28A and LIN28B are upregulated in primary human tumors and in human cancer cell lines, their overexpression directly correlating to downregulation of let-7 miRNAs (4). LIN28 genes are reported to be involved in primordial germ cell development and germ cell malignancy (5), and allelic variation in LIN28B is associated with regulating the timing of puberty in humans (6). Overexpression of LIN28A, in conjunction with Oct-4, Sox2 and Nanog, can reprogram human fibroblasts to pluripotent, ES-like cells (7).\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

L2279-95

Size

100ul

Applications

WB

Hosts

Rabbit

Reactivities

Mouse

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

(1) Balzer, E. and Moss, E.G. (2007) RNA Biol 4, 16-25. (2) Piskounova, E. et al. (2008) J Biol Chem 283, 21310-4. (3) Cho, W.C. (2007) Mol Cancer 6, 60. (4) Viswanathan, S.R. et al. (2009) Nat Genet 41, 843-8. (5) West, J.A. et al. (2009) Nature 460, 909-13. (6) Ong, K.K. et al. (2009) Nat Genet 41, 729-33. (7) Yu, J. et al. (2007) Science 318, 1917-20.

Additional Info

Recognizes endogenous levels of total mouse LIN28B protein.

Alternative Names

Lin-28 Homolog B (C. elegans), 2810403D23Rik, D030047M17Rik