MAdCAM-1 is a key player in mediating the infiltration of leukocytes into chronically inflamed tissue. MAdCAM-1 is a cell-surface Ig superfamily member composed of two extracellular Ig domains, followed by a mucin-like domain, a transmembrane domain and a short cytoplasmatic domain. It interacts via its N-terminal Ig domain with the lymphocyte homing receptor alpha4beta7, which plays a critical role in forming the gut-associated lymphoid system. MAdCAM-1 promotes the adhesion of T- and B cells, monocytes/macrophages, and potentially eosinophils, basophils, and differentiated mast cells to the vascular endothelium. Mucosal addressin cell adhesion molecule-1 RNA transcripts are predominantly expressed in the small intestine, mesenteric lymph nodes, colon and spleen; and are very weakly expresssed in human pancreas and brain. This monoclonal antibody detects MAdCAM-1 on venules in the spleen and small intestine. MAdCAM-1 is strongly expressed in the synovium of osteoarthritis patients, predominantly on the endothelial lining of blood vessels, but also within the vessel lumen. This monoclonal antibody may be useful in diagnosis of inflammation in humans by monitoring the presence and levels of MAdCAM-1.
Applications:
Suitable for use in Flow Cytometry, ELISA, Western Blot, Immunohistochemistry and Inhibition of biological activity. Other applications not tested.
Recommended Dilution:
Immunohistochemistry: Frozen
Immunohistochemistry: Paraffin
Flow Cytometry: 1:50
Western Blot: 1:50
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.
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