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Mannan Binding Protein (MBP, Mannose Binding Protein, Mannan Binding Lectin, MBL)

Cat no: M2250-06

Mannan Binding Protein (MBP, Mannose Binding Protein, Mannan Binding Lectin, MBL)

Mannan-binding lectin (MBL) belongs to the C-type family of collectins. On specific binding to microbial surface carbohydrates, MBL activates the complement system by means of its own lectin pathway, depending on the MBL-associated serine proteases (MASPs). Investigations have shown that up to 12% of normal blood donors (1) have low (<50ng/ml) MBL plasma concentrations.\n\nApplications: \nSuitable for use in ELISA, Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:10000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

M2250-06

Size

100ul

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2, 15mM sodium azide.\n

P Type

Mab

Purity

Purified by Protein A/G affinity chromatography from culture supernatant.

Isotype

IgG1,k

References

1. Steffensen R, Thiel S, Varming K, Jersild C, Jensenius JC (2000) Detection of structural gene mutations and promoter polymorphisms in the mannan-binding lectin (MBL) gene by polymerase chain reaction with sequence-specific primers. J Immunol Methods 241:33-42.\n2. Kawasaki N, Kawasaki T, Yamashina I (1983) Isolation and characterization of a mannan-binding protein from human serum. J Biochem (Tokyo) 94:937-947.\n3. Madsen J, Tornoe I, Nielsen O, Koch C, Steinhilber W, Holmskov U (2003) Expression and localization of lung surfactant protein A in human tissues. Am J Respir Cell Mol Biol 29:591-7.\n4. Holmskov UL (2000) Collectins and collectin receptors in innate immunity. APMIS Suppl 100:1-59.\n5. Harboe M, Garred P, Borgen MS, Stahl GL, Roos A, Mollnes TE (2006) Design of a complement mannose-binding lectin pathway-specific activation system applicable at low serum dilutions. Clin Exp Immunol 144:512-520.

Additional Info

Specific for MBL from human serum or plasma. Epitope specificity differs from those of clones 6F140 and 6F141.\nReacts with human MBL both in its polymeric state and as a single subunit of molecular mass 26kD.

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