MDM2 (E3 Ubiquitin-protein Ligase Mdm2, Double Minute 2 Protein, Hdm2, Oncoprotein Mdm2, p53-binding Protein Mdm2)

The MDM2 (murine double minute 2) proto-oncogene was originally identified as an amplified gene in a mouse tumor cell line. Overexpression of MDM2 produces tumors in athymic mice and binds to and inactivates the transcriptional activity of the p53 protein. p53 was identified as the major target for MDM2 during embryonic development. The MDM2 gene itself is a transcriptional target for p53, and induction of p53 transcriptional activity results in increased MDM2 mRNA and protein levels. It appears that a MDM2 p53 feedback loop serves to keep the growth suppressive functions of p53 in check during the normal cell cycle. MDM2 regulates cell proliferation via p53-independent pathways and can interact with Rb, E2F-1 and DP1. Because MDM2 can bind to and inactivate the transcriptional activity of p53, Overexpression has been detected in a variety of human tumors, and appears to result from gene amplification, increased transcript level, and enhanced translation.\n\nApplications: \nSuitable for use in Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry: 1:50-1:100\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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