MECP2, phosphorylated (Ser292) (Methyl-CpG-binding Protein 2, MeCP-2 Protein, MeCP2) (FITC)

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of some cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females.\n\nApplications: \nSuitable for use in ELISA and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nELISA: 1:1000\nImmunohistochemistry: 1:50-1:100\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze FITC conjugates. Light sensitive. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

M2763-30A-FITC

Size

200ul

Applications

ELISA, IHC

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2. Contains ~0.09% sodium azide. Conjugated to FITC.

P Type

Pab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG

References

1.Mnatzakanian, G.N., et al., Nat. Genet. 36(4):339-341 (2004). 2.Laccone, F., et al., Hum. Mutat. 23(3):234-244 (2004). 3.Suzuki, M., et al., Oncogene 22(54):8688-8698 (2003). 4.Balmer, D., et al., J. Mol. Med. 81(1):61-68 (2003). 5.Hagberg, B., et al., Eur. J. Paediatr. Neurol. 7(6):417-421 (2003).

Additional Info

Recognizes human MECP2 when phosphorylated at Ser292.