Microphthalmia (Transcription Factor, MITF)

MITF (Microphthalmia-associated transcription factor) is a member of the MiT/TFE family of molecules. Although is has a predicted MW of 58kD, it runs anomalously in SDS-PAGE at 57-66kD. MITF-A1 is found in melanocytes and RPEs where it regulates melanin synthesis via tyrosinase and TRP-1 gene expression. Human MITF-A1 is 526aa in length. It contains a bHLH DNA binding region (aa309-369) and a Leu-zipper domain (aa374-395). There are three phosphorylation sites at Ser54/405/414, and two SUMOylation sites at Arg289/423. MITF-A1 acts as either a homodimer, or heterodimer with TFE3, TFEB or TFEC. Multiple splice variants exist, with each variant expressing one of two isoforms that are defined by the presence (#1), or absence (#2), of aa294-299. One variant has a deletion of aa139-194, a second has an 11aa substitution for aa1-118, and three others show variable substitutions over aa1-35. Over aa119-289, human MITF-A1 shares 96% aa identity with mouse MITF-A1.\n\nApplications:\nSuitable for use in ELISA, Immunohistochemistry, and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1ug/ml\nELISA: 0.5-1ug/ml\nImmunohistochemistry: 3-10ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS, sterile 40-50% glycerol, aliquot and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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