MP1 (MEK partner 1)

The function of MP1 (MEK partner-1) a 14kD scaffold protein, is to bind specifically to MEK1 and ERK1 and bring them in close proximity in order to facilitate their activation (1). Localized to the late endosome, MP1 also interacts with an adaptor protein p14. While the interaction between MP1-ERK1 can take place in the absence of the adaptor protein p14, it is strengthened in the presence of p14 (2). Additionally, it has been suggested that the binding of MP1 to MEK1resides in a proline-rich sequence (residue 270-307) of MEK1\n\nApplications: \nSuitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:10,000-50,000\nImmunohistochemistry: 1:100-250\nImmunocytochemistry: 1:50-100\nImmunoprecipitation: 1:50\n\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Freeze at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. \n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

M4679-50

Size

100ul

Applications

ICC, IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1. Schaeffer, H. J., Catling, A. D., Eblen, S. T., Collier, L. S., Krauss, A., and Weber, M. J. (1998) Science 281, 1668-1671 2. Teis, D., Wunderlich, W., and Huber, L. A. (2002) Dev. Cell3, 803-814

Additional Info

Recognizes human mitogen-activated protein kinase kinase 1 interacting protein 1