Myh9l2, CT (Myh9l2 Protein, zgc:162029)

Nonmuscle myosin class II proteins belong to the superfamily of actin- based motor proteins. They form hexamer structures which compose of two ~200kD heavy chains (NMHC II) and two pairs of 20kD and 17kD light chains (MLC20 and MLC17). Nonmuscle myosins are classified according to the heavy chain isoforms; light chains are conserved. Three different isoforms, named NMHC II-A, NMHC II-B, and NMHC II-C, respectively, that share 64-80% aa sequence identity, have been detected in mammals. Although NMHC II molecules have now been found in muscle cells as well, the name "nonmuscle myosin" was already widespread and is in use today. Zebrafish myosin heavy chain 9 non-muscle like-2 (Myh9l2) is correspond to the NMHC II in the mammalian animals and comparison studies with human MYHs suggests a divergent evolutionary lineage of fish and tetrapod MYHs.\n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:5000-1:20,000\nWestern Blot: 1:500-1:1000\nOptimal dilutions to be determined by the researcher.\n\nControl Peptide: M9848-19P\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

M9848-19

Size

250ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

ZF/Fish

Form

Supplied as a liquid in 40mM MOPS, pH 7.5, 0.1% BSA, 0.05% sodium azide, 50% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Golomb E. et al. (2004) J. Biol. Chem. 279(4): 2800-2808. 2. Conti M. A. et al. (2007) J. of Cell Sci. 121: 11-18. 3. Ikeda D. et al. (2007) Physiol Genomics 32:1-15.

Additional Info

Recognizes zebrafish Myh9a.