Myosin IIA, Non-muscle, CT (Myosin-9, Myosin Heavy Chain 9, Myosin Heavy Chain, Non-Muscle IIa, Non-Muscle Myosin Heavy Chain IIa, NMMHC II-a, NMMHC-IIA, Cellular Myosin Heavy Chain, Type A, Non-Muscle Myosin Heavy Chain A, NMMHC-A, Myh9)

Nonmuscle myosin class II proteins belong to the superfamily of actin-based motor proteins. They form hexamer structures composed of two ~200kD heavy chains (NMHC II) and two pairs of 20kD and 17kD light chains (MLC20 and MLC17). Nonmuscle myosins are classified according to the heavy chain isoforms; light chains are conserved. Three different isoforms that share 64-80% aa sequence identity have been detected in mammals: NMHC II-A, NMHC II-B, and NMHC II-C. Although NMHC II molecules have now been found in muscle cells as well, the name "nonmuscle myosin" was already widespread and is in use today. NMHC II-A is most abundant compared to other NMHC isoforms in human lung, spleen, thymus, uterus, and colon. Thus, single aa mutation in MNMH II-A results in kidney and platelet defects in humans. In addition, recent study indicates that replacement of NMHC II-B with NMHC II-A rescues brain defect in mice.\n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.5-2ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

M9848-12B

Size

50ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.05% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Golomb E. et al. (2004) J. Biol. Chem. 279(4): 2800- 2808. 2. Conti M. A. et al. (2007) J. of Cell Sci. 121: 11-18. 3. Bao J. e 282(30): 3. Bao J. et al. (2007) J. Biol. Chem. 22102-22111.

Additional Info

Recognizes human NMHC II-A.