Myotubularin Related Protein 4, CT (MTMR4, FYVE Domain-containing Dual Specificity Protein Phosphatase 2, FYVE-DSP2, KIAA0647, Zinc Finger FYVE Domain-containing Protein 11, ZFYVE11)

MTMR, a member of the myotubularin family, encodes an intracellular protein of 1,195 amino acids, and shares approximately 47% sequence identity with MTMR3, spiking to over 70% identity in the catalytic domain and the double zinc finger (FYVE) motif. PCR analysis detects expression of MTMR4 in all human tissues examined except lung, small intestine, stomach, salivary gland, adrenal gland, and uterus. Subcellular localization of MTMR4 in transfected HeLa cells showed a perinuclear distribution, similar to that seen with MTMR3. MTMR4 dephosphorylates para-nitrophenylphosphate and phosphatidylinositol 3-phosphate.\n\nApplications: \nSuitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:1,000\nWestern Blot: 1:100-1:500\nImmunohistochemistry: 1:10-1:50\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

M9887-08C

Size

200ul

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, 0.09% sodium azide.

P Type

Pab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG

References

1. Junjing Yu, et al. J. Biol. Chem., Mar 2010; 285: 8454-8462 (Applications: WB)(E-pub). 2. Yu J, et al. J Biol Chem. 2010 Mar 12;285(11):8454-62. (E-pub).

Additional Info

Recognizes human MTMR4. Species Crossreactivity: mouse.

Alternative Names

Myotubularin-related Protein 4, EC=3.1.3.48