Nitric Oxide Colorimetric BioAssay(TM) Kit

Nitric oxide (NO) plays an important role in neurotransmission, vascular regulation, immune responses and apoptosis. Since most of the NO is oxidized to nitrite (NO2-) and nitrate (NO3-), the concentrations of these anions have been used as a quantitative measure of NO production. N2577-01 provides an accurate and convenient measurement of total nitrate/nitrite concentration in a simple two-step process. The first step is to convert nitrate to nitrite utilizing nitrate reductase. The second step involves addition of the Griess Reagents which convent nitrite into a deep purple azo compound. Measurement of the absorbance of the azo chromophore accurately determines the total nitric oxide production. Sample Matrix: Cell and Tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids Detection Limit: The detection limit of the assay is 0.1nmole/well, or ~1uM. Kit Components: N2577-01A: Assay Buffer, 1x30ml N2577-01B: Enzyme Co-factors, 1vial N2577-01C: Nitrate Reductase: 1vial N2577-01D: Nitrate Standard, 1vial N2577-01E: Griess Reagent R1, 1x10ml N2577-01F: Griess Reagent R2, 1x10ml N2577-01G: Microwell Plate, 2x96assays N2577-01H: Plate Cover, 2x1cover N2577-01J: Enhancer 1vial N2577-01K: Nitrite Standard: 1vial Storage and Stability: N2577-01A, Assay Buffer: Store at 4 degrees C. N2577-01B, Enzyme Co-factors: Store at -20 degrees C. After reconstitution, aliquot and store at -20 degrees C. Keep on ice during use. N2577-01C, Nitrate Reductase: Store at -20 degrees C.After reconstitution, aliquot and store at -20 degrees C. Keep on ice during use. N2577-01D, Nitrate Standards: Store at -20 degrees C. After reconstitution, store at 4 degrees C. Do not freeze! The reconstituted standard is stable for 4 months when stored at 4 degrees C. N2577-01E, Griess Reagent R1, and N2577-01F, Griess Reagent R2: Ready to use. Store at 4 degrees C. N2577-01J, Enhancer: Store at -20 degrees C. After reconstitution, store at 4 degrees C. N2577-01K, Nitrite Standard: Store at -20 degrees C. After reconstitution, store at 4 degrees C. Do not freeze! The reconstituted standard is stable for 4 months when stored at 4 degrees C. Kit is stable for 4 months with proper storage and handling.