Nitric Oxide Colorimetric BioAssay(TM) Kit

Nitric oxide (NO) plays an important role in neurotransmission, vascular regulation, immune responses and apoptosis. Since most of the NO is oxidized to nitrite (NO2-) and nitrate (NO3-), the concentrations of these anions have been used as a quantitative measure of NO production. N2577-01 provides an accurate and convenient measurement of total nitrate/nitrite concentration in a simple two-step process. The first step is to convert nitrate to nitrite utilizing nitrate reductase. The second step involves addition of the Griess Reagents which convent nitrite into a deep purple azo compound. Measurement of the absorbance of the azo chromophore accurately determines the total nitric oxide production.\n\nSample Matrix:\nCell and Tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids \n\nDetection Limit:\nThe detection limit of the assay is 0.1nmole/well, or ~1uM. \n \nKit Components:\nN2577-01A: Assay Buffer, 1x30ml\nN2577-01B: Enzyme Co-factors, 1vial\nN2577-01C: Nitrate Reductase: 1vial\nN2577-01D: Nitrate Standard, 1vial\nN2577-01E: Griess Reagent R1, 1x10ml\nN2577-01F: Griess Reagent R2, 1x10ml\nN2577-01G: Microwell Plate, 2x96assays\nN2577-01H: Plate Cover, 2x1cover\nN2577-01J: Enhancer 1vial\nN2577-01K: Nitrite Standard: 1vial\n\nStorage and Stability:\nN2577-01A, Assay Buffer: Store at 4 degrees C. \nN2577-01B, Enzyme Co-factors: Store at -20 degrees C. After reconstitution, aliquot and store at -20 degrees C. Keep on ice during use.\nN2577-01C, Nitrate Reductase: Store at -20 degrees C.After reconstitution, aliquot and store at -20 degrees C. Keep on ice during use.\nN2577-01D, Nitrate Standards: Store at -20 degrees C. After reconstitution, store at 4 degrees C. Do not freeze! The reconstituted standard is stable for 4 months when stored at 4 degrees C.\nN2577-01E, Griess Reagent R1, and N2577-01F, Griess Reagent R2: Ready to use. Store at 4 degrees C.\nN2577-01J, Enhancer: Store at -20 degrees C. After reconstitution, store at 4 degrees C.\nN2577-01K, Nitrite Standard: Store at -20 degrees C. After reconstitution, store at 4 degrees C. Do not freeze! The reconstituted standard is stable for 4 months when stored at 4 degrees C.\n\nKit is stable for 4 months with proper storage and handling.

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SPECIFICATIONS

Catalog Number

N2577-01

Size

2Plates

References

US Biological application references: 1. Hu, J., et al., Neuroscience 151: 680-691 (2008). 2. Wu, C.-Y., et al., Neuroscience 158: 1500-1508 (2009). 3. Dheen, S.T., et al., Glia 50: 21-31 (2004). 4. Zou, Y.Y., et al., Neuroscience 160: 698-709 (2009). 5. Kaur, C., et al., Investigative Ophthalmology and Visual Science 50: 5364-5374 (2009). 6. Kaur, C., et al., J. Pineal. Research 48: 185-193 (2010). 7. Zou, Y.-Y., et al., (2012) Neuropathology and Applied Neurobiology ,DOI: 10.1111/nan.12001.